| 產品編號 | IHC0107 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 英文名稱 | MBP Ready-To-Use IHC Kit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 中文名稱 | 髓鞘堿性蛋白/磷脂堿性蛋白即用型免疫組化試劑盒 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 別 名 | Myelin Basic Protein; Myelin basic protien; GDB; Golli MBP; Hemopoietic MBP; HMBPR; HUGO; MBP; MGC99675; MLD; Myelin A1 Protein; Myelin Deficient; Myelin Membrane Encephalitogenic Protein; SHI; Shiverer; SP; MBP_HUMAN . | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 種 屬 | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 應用范圍 | Immunohistochemistry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 適用樣品基質 | PFPE tissue | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 描述 | IHC0107 is a ready-to-use IHC kit for staining of MBP. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 保存條件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 產品介紹 |
產品組分及規格
背景 The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
石蠟包埋組織的免疫組織化學方案 1. 脫蠟水化 石蠟切片置于新鮮二甲苯中浸泡脫蠟3次，每次15 min；依次置于不同濃度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸餾水洗滌5 min，重復3次。 2. 抗原修復 沸水浴修復：將100×抗原修復緩沖液（試劑2）用蒸餾水稀釋成1×抗原修復緩沖液，放入修復盒中并提前加熱至95-100℃（注意蓋好以防液體蒸發），然后將切片放入修復盒中，在沸水浴環境中保持外沸狀態15 min，室溫自然冷卻；用PBS緩沖液（試劑1，將干粉溶解在2L蒸餾水中）清洗5 min，重復3次。 3. 阻斷內源性過氧化物酶 用吸水紙吸去玻片上多余的液體，用免疫組化筆在組織周圍畫圈，加入2-4滴內源性過氧化物酶阻斷劑（試劑3），室溫下置于濕盒中孵育15 min，用PBS洗滌5 min，重復3次。 4. 血清封閉 用吸水紙吸去玻片上多余的液體，加入2-4滴封閉工作液（試劑4），置于濕盒內37℃封閉20 min，以減少非特異性染色。 5. 一抗孵育 用吸水紙吸去玻片上多余的液體，加入2-4滴MBP 兔多抗工作液（試劑5），置于濕盒中，4℃孵育過夜或37℃孵育1-2 h。 6. 復溫 4℃孵育過夜后，室溫下復溫15 min (若在室溫下孵育一抗，則直接進入下一步清洗)；用PBS洗滌5 min，重復3次。 7. 二抗孵育 用吸水紙吸玻片上多余的液體，加入2-4滴HRP標記羊抗兔IgG工作液（試劑6），置于濕盒中，37℃孵育1-2 h；用PBS洗滌5 min，重復3次。 8. 顯色 用吸水紙吸去玻片上多余的液體，在每張切片上滴加約50 μL新配制的DAB工作液（試劑7:試劑8:PBS=1:1:18），作用3-5 min。顯微鏡下觀察結果，達到合適的顯色強度后，用蒸餾水沖洗切片以終止反應，用蒸餾水沖洗5 min，重復3次。 9. 復染 滴加適量復染試劑（試劑9）復染3-5 min，蒸餾水沖洗5 min，滴加鹽酸酒精分化約30 s，蒸餾水洗滌5 min，重復2次。 10. 脫水封片 將玻片依次置于不同濃度（70%、80%、90%、95%、100%）乙醇，各5 min；然后置于新鮮二甲苯中浸泡脫蠟3次，每次15 min。用吸水紙吸去多余的二甲苯，滴加適量封片劑（試劑10）在組織上，將蓋玻片蓋在組織上，避免產生氣泡。
注意事項 1. 建議檢測時進行陰性及陽性對照，以提高實驗的可靠性。 2. 本品中的配套試劑，請不要用其他生產商產品替換使用。 3. DAB為致癌物質，請采取必要的防范措施。 4. PBS洗滌液（試劑1）配制后在4℃可保存一周；抗原修復液（試劑2）及顯色劑（試劑7和8）的工作液需每次實驗時現用現配。 *5. 發表論文時引用本產品的寫作建議 "IHC0107, Bioss Antibodies"。引用示例: “Tissue sections using MBP IHC Kit (IHC0107, Bioss Antibodies) were stained for MBP according to the manufacturer's instructions.” |
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| 產品圖片 |
Immunohistochemical analysis of paraffin embedded mouse brain tissue slide using IHC0107 (MBP IHC Kit).
Immunohistochemical analysis of paraffin embedded rat brain tissue slide using IHC0107 (MBP IHC Kit).
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Immunohistochemical analysis of paraffin embedded human brain tissue slide using IHC0107 (MBP IHC Kit).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
