| 产品编号 | bsm-60711R |
| 英文名称 | Rabbit Anti-phospho-IKB Alpha (Ser32 / Ser36) antibody |
| 中文名称 | 磷酸化核因子κB抑制蛋白α重组兔单抗 |
| 别 名 | NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN; IκB-α; IκBα. |
| 产品类型 | 磷酸化抗体 重组兔单抗 |
| 研究领域 | 细胞表面分子 淋巴细胞 b-淋巴细胞 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | R8D3 |
| 交叉反应 | (predicted: Human,Mouse,Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:50-100,IF=1:1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human phospho-IKB Alpha (Ser32 / Ser36) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Highly expressed in lymph node, thymus followed by liver, brain, muscle, kidney, gastrointestinal and reproductive tract. SWISS: Q9Z1E3 Gene ID: 4792 Database links: Entrez Gene: 4792 Human Entrez Gene: 18035 Mouse SwissProt: P25963 Human SwissProt: Q9Z1E3 Mouse |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: (-): C6, (+): C6+ Calyculin A (100nM,
30min)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 36 kDa
Observed MW: 36 kDa
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: (-): HeLa, (+): HeLa+ Human 伪-TNF
(20ng/ml, 30min)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 36 kDa
Observed MW: 36 kDa
Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline
phosphatase (50 U/ml) at 37℃ for 1 hour
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60711R
Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50
U/ml) at 37℃ for 1 hour
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
bsm-60711R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
