| 产品编号 | bsm-60637R |
| 英文名称 | Rabbit Anti-Claudin 18.2 antibody |
| 中文名称 | 紧密连接蛋白18重组兔单抗 |
| 别 名 | Claudin-18; CLD18_HUMAN; CLDN18; SFTA5; SFTPJ; UNQ778/PRO1572. |
| 研究领域 | 细胞生物 信号转导 转运蛋白 细胞粘附分子 内皮细胞 跨膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | R7G12 |
| 交叉反应 | Human,Rat (predicted: Mouse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Claudin 18 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Claudin superfamily consists of many structurally related proteins in humans (1). These proteins are important structural and functional components of tight junctions in paracellular transport (1,2). Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues (1). Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and Junction adhesion molecule (3). Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands (4). Emerging evidence suggests that the Claudin family of proteins regulates transport through tight junctions via differential discrimination for solute size and charge (5).Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions (6). SWISS: P56856 Gene ID: 51208 Database links: Entrez Gene: 51208 Human Entrez Gene: 56492 Mouse Omim: 609210 Human SwissProt: P56856 Human SwissProt: P56857 Mouse Unigene: 655324 Human Unigene: 386784 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium EDTA buffer (9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 18.2) Monoclonal Antibody, Unconjugated (bsm-60637R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 18.2) Monoclonal Antibody, Unconjugated (bsm-60637R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium EDTA buffer (9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 18.2) Monoclonal Antibody, Unconjugated (bsm-60637R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
