| 产品编号 | bsm-60067M |
| 英文名称 | Mouse Anti-Acetyl-Histone H4 (Lys8) antibody |
| 中文名称 | |
| 产品类型 | 乙?；固?/a> |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | B11C10 |
| 交叉反应 | (predicted: Human,Mouse,Rat) |
| 产品应用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG1/Kappa |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 组蛋白翻译后修饰（PTMs）是表观遗传学调控染色质结构的关键机制，被称为“组蛋白密码”。组蛋白上的PTMs包括乙?；⒓谆?、磷酸化和新的?；苯佑跋烊旧识宰家蜃拥目杉靶院推渌砉垡糯骺兀谋浠蜃榈奈榷ㄐ?，基因转录等。组蛋白乙?；饕⑸诤诵淖榈鞍装被┒说亩喔隼蛋彼岵谢希韵煊Ω髦执碳?，在基因表达调控、DNA损伤修复、染色质动力学等方面发挥重要作用。大多数情况下，组蛋白H2A主要在Lys5、9、15和36位处发生乙酰化修饰。 H2B主要在Lys5、12、15、16和20位处发生乙?；奘?。组蛋白H3主要在Lys4、9、14、18、23、27、56和79位处发生乙?；奘?。组蛋白H4主要在Lys5、8、12、16和20位处发生乙?；奘巍Ｗ榈鞍滓阴Ｗ泼福℉ATs）和组蛋白去乙酰化酶（HDACs）是主要的调节因子。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+SBA (5mM, 24hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Mouse kidney
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60067M
Tissue: Human hepatocellular carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60067M
Tissue: Rat liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60067M
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
