| 产品编号 | bs-60036R |
| 英文名称 | Rabbit Anti-Acetyl-Histone H4 (Lys16) antibody |
| 中文名称 | |
| 产品类型 | 乙酰化抗体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rice) |
| 产品应用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | Antigen affinity purification |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 组蛋白翻译后修饰（PTMs）是表观遗传学调控染色质结构的关键机制，被称为“组蛋白密码”。组蛋白上的PTMs包括乙酰化、甲基化、磷酸化和新的酰化直接影响染色质对转录因子的可及性和其他表观遗传调控，改变基因组的稳定性，基因转录等。组蛋白乙酰化主要发生在核心组蛋白氨基末端的多个赖氨酸残基上，以响应各种刺激，在基因表达调控、DNA损伤修复、染色质动力学等方面发挥重要作用。大多数情况下，组蛋白H2A主要在Lys5、9、15和36位处发生乙酰化修饰。 H2B主要在Lys5、12、15、16和20位处发生乙酰化修饰。组蛋白H3主要在Lys4、9、14、18、23、27、56和79位处发生乙酰化修饰。组蛋白H4主要在Lys5、8、12、16和20位处发生乙酰化修饰。组蛋白乙酰转移酶（HATs）和组蛋白去乙酰化酶（HDACs）是主要的调节因子。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:400
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Rice
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human neuroblastoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:200
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bs-60036R
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:200
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bs-60036R
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
