| 產品編號 | bsm-41304M |
| 英文名稱 | CD80 Mouse mAb |
| 中文名稱 | 刺激分子B7-1蛋白單克隆抗體 |
| 別 名 | Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen(CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80. |
| 研究領域 | 腫瘤 免疫學 干細胞 淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 11B12 |
| 交叉反應 | Human |
| 產品應用 | Flow-Cyt=1ug/Test,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant mouse CD80 protein: 38-246/306 |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011] SWISS: Q00609 Gene ID: 12519 Database links: Entrez Gene: 941 Human Entrez Gene: 12519 Mouse Omim: 112203 Human SwissProt: P33681 Human SwissProt: Q00609 Mouse Unigene: 838 Human Unigene: 89474 Mouse |
| 產品圖片 |
Raji cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CD80) monoclonal Antibody, Unconjugated (bsm-41304M) 1:50, 90 minutes at 37°C; fol
Blank control:Raji.
Primary Antibody (green line): Mouse Anti-CD80 antibody (bsm-41304M)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-mouse IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protoco
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
