| 产品编号 | bsm-41731M |
| 英文名称 | Mouse Anti-Mucin-1 antibody |
| 中文名称 | 粘蛋白-1单克隆抗体 |
| 别 名 | Breast carcinoma associated antigen DF3; Breast carcinoma associated antigen DF3; Breast carcinoma-associated antigen DF3; CA 15 3; CA 15-3; CA15 3; CA15 3 antigen; CA15.3; Cancer antigen 15-3; Carcinoma associated mucin; Carcinoma associated mucin; Carcinoma-associated mucin; CD 227; CD227; CD227; CD227 antigen; DF3 antigen; DF3 antigen; EMA; EMA; Episialin; Episialin; Epithelial membrane antigen; Epithelial membrane antigen; Epithelial mucin tandem repeat sequence; H23 antigen; H23 antigen; H23AG; H23AG; HGNC:7508; KL 6; KL-6; KL6; Krebs von den Lungen-6; MAM 6; MAM6; MUC 1; MUC 1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1; MUC1-alpha; MUC1-beta; MUC1-CT; MUC1-NT; MUC1/ZD; MUC1_HUMAN; Mucin 1; Mucin 1 precursor; Mucin 1 transmembrane; Mucin-1 subunit beta; Mucin1; Mucin1; Peanut reactive urinary mucin; Peanut reactive urinary mucin; Peanut-reactive urinary mucin; PEM; PEMT; PEMT; Polymorphic epithelial mucin; Polymorphic epithelial mucin; PUM; PUM; Tumor associated epithelial membrane antigen; Tumor associated epithelial membrane antigen; Tumor associated epithelial mucin; Tumor associated mucin; Tumor associated mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin. |
| 研究领域 | 肿瘤 细胞生物 t-淋巴细胞 上皮细胞 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 6E9 |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 138kDa |
| 细胞定位 | 细胞核 细胞浆 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant Human Mucin-1 Protein: 921-1158/1255 |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. SWISS: P15941 Gene ID: 4582 Database links: Entrez Gene: 4582 Human Entrez Gene: 17829 Mouse Omim: 158340 Human SwissProt: P15941 Human SwissProt: Q02496 Mouse Unigene: 89603 Human Unigene: 16193 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Mucin-1) Monoclonal Antibody, Unconjugated (bsm-41731M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Mucin-1) Monoclonal Antibody, Unconjugated (bsm-41731M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Mucin-1) Monoclonal Antibody, Unconjugated (bsm-41731M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Mucin-1) Monoclonal Antibody, Unconjugated (bsm-41731M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Mucin-1) Monoclonal Antibody, Unconjugated (bsm-41731M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
