| 产品编号 | bs-80295G-HRP |
| 英文名称 | Goat Anti-Rabbit IgG H&L antibody |
| 中文名称 | 辣根过氧化物酶标记羊抗兔IgG (H+L) |
| 别 名 | Goat Anti-Rabbit IgG H&L /HRP; Immunoglobulin G; 辣根过氧化物酶标记二抗; 辣根过氧化物酶标记山羊抗兔IgG (H+L); HRP标记山羊抗兔IgG (H+L); HRP标记山羊抗兔IgG (H+L); |
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Specific References (6) | bs-80295G-HRP has been referenced in 6 publications.
[IF=10.588] Haiyang Zhang. et al. Co-exposure of chronic stress and alumina nanoparticles aggravates hippocampal microglia pyroptosis by activating cathepsin B/NLRP3 signaling pathway. J HAZARD MATER. 2022 Aug;436:129093 IHC ; Rat.
[IF=7.419] Abulaiti Abulizi. et al. Quince extract resists atherosclerosis in rats by down-regulating the EGFR/PI3K/Akt/GSK-3β pathway. BIOMED PHARMACOTHER. 2023 Apr;160:114330 WB ; Rat.
[IF=6.268] Guo, Xin. et al. The histone demthylase KDM3A protects the myocardium from ischemia/reperfusion injury via promotion of ETS1 expression. Commun Biol. 2022 Mar;5(1):1-15 WB ; Rat.
[IF=4.136] Xiaoyue Huang. et al. Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma. ONCOL REP. 2022 Jul;48(1):1-18 WB ; Human.
[IF=3.616] Shugen Xu. et al. Effect of miR-21-3p on lung injury in rats with traumatic hemorrhagic shock resuscitated with sodium bicarbonate Ringer’s solution. ANN TRANSL MED. 2022 Dec; 10(24): 1331 WB ; Rat.
[IF=2.586] Peng Zhang. et al. Effect of isorhamnetin on carbonic anhydrase IX expression and tumorigenesis of bladder cancer by activating PPARγ/PTEN/AKT pathway. TISSUE CELL. 2023 Jun;82:102048 IHC,WB ; Mouse,Human.
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| 抗体来源 | Goat |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rabbit |
| 产品应用 | WB=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 1.0 mg/ml |
| 免 疫 原 | Native Rabbit IgG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
HRP Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed
HRP antibodies can be used for a variety of applications, including ELISA, Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.
Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and ELISA applications. |
| 产品图片 |
Sample:
Lane 1: Mouse Spleen Lysates
Lane 2: Human SH-SY5Y cell Lysates
Primary: Anti-beta-Actin (bs-0061R) at 1/1000 dilution
Secondary: Goat Anti-Rabbit IgG H&L/HRP at 1/100000 dilution
Predicted band size: 42kDa
Observed band size: 45kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
