| 产品编号 | bs-55098R |
| 英文名称 | Rabbit Anti-HMGB1 antibody |
| 中文名称 | 高迁移率族蛋白B1抗体 |
| 别 名 | High mobility group protein B1; Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; HMG3; HMGB 1; HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-1000,IHC-P=1:50-100,IHC-F=1:400-800,IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HMGB1: 100-200/215 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Subunit: Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Subcellular Location: Nucleus. Chromosome. Similarity: Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains. SWISS: P09429 Gene ID: 3146 Database links: Entrez Gene: 3146 Human Entrez Gene: 100862258 Mouse Entrez Gene: 15289 Mouse Omim: 163905 Human SwissProt: P09429 Human 近来的研究表明称之为高迁移率族蛋白B-1（HMG-B1）的核内结构蛋白在核外表达时是一种有效的早期炎症介质。 高迁移性Ｂ１组蛋白（ＨＭＧＢ１）： 是一种核结合蛋白，在ＤＮＡ重组、修复、复制和基因转录中起作用。ＨＭＧＢ１也是巨噬细胞分泌的一种介质。 此外，高迁移性Ｂ１组蛋白亦被受刺激的巨噬细胞或单核细胞主动分泌。在这一主动分泌过程中，HMGB1首先经乙酰化并由核内转移至溶酶体内，继而在ATP和溶血磷脂胆碱两种分泌信号指导下转移至细胞外。由坏死细胞被动释放的HMGB1和炎症细胞主动分泌的HMGB1存在分子上的差异。胞外的HMGB1可作为细胞因子参与信号传导，因为它既可识别Toll 样受体（TLR）家族的一些成员，又能与识别晚期糖基化终末产物受体（RAGE）相作用。 HMGB1能启动炎症反应，包括产生多种细胞因子、对某些干细胞产生趋化作用、诱导血管粘附分子、削弱肠上皮细胞的功能等 |
| 产品图片 |
Sample:
Lane 1: Human Hela Cell (Control) Lysate
Lane 2: HMGB1 knockout (KO) HeLa Cell Lysate
Primary: Anti-HMGB1 (bs-55098R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 30 kD
Sample:
Lane 1: Lung (Mouse) TissueLysate at 40 ug
Lane 2: Liver (Mouse) Tissue Lysate at 40 ug
Lane 3: Testis (Rat) Tissue Lysate at 40 ug
Lane 4: Cerebrum (Rat) Tissue Lysate at 40 ug
Lane 5: Jurkat (Human) Cell Lysate at 30 ug
Lane 6: Hela (Human) Cell Lysate at 30 ug
Lane 7: HepG2 (Human) Cell Lysate at 30 ug
Lane 8: Molt-4 (Human) Cell Lysate at 30 ug
Primary:
Anti-HMGB1 (bs-55098R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-55098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
