| 产品编号 | bsm-33226M |
| 英文名称 | Mouse Anti-RuBisCO antibody |
| 中文名称 | 核糖二磷酸羧化酶大链单克隆抗体 |
| 别 名 | rbcL; RBL_SPIOL; Ribulose bisphosphate carboxylase large chain; RuBisCO-large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit. |
| 产品类型 | 植物抗体 |
| 研究领域 | 肿瘤 细胞生物 信号转导 植物 新陈代谢 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 3G5 |
| 交叉反应 | Arabidopsis Thaliana |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | D-Ribulose 1,5-Diphosphate Carboxylase purified |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. Function: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. Subunit: Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers. Subcellular Location: Plastid, chloroplast. Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization. Similarity: Belongs to the RuBisCO large chain family. Type I subfamily. SWISS: P00875 Gene ID: 2715621 Database links: Entrez Gene: 2715621 Spinacia oleracea SwissProt: P00875 Spinacia oleracea
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| 产品图片 |
Sample:
Lane 1: Brassica oleracea L. leaves (Plant) lysates
Lane 2: Chinese cabbage leaves (Plant) lysates
Primary: Anti-RuBisCO (bsm-33226M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
