| 产品编号 | bs-40295G-IRDye8 |
| 英文名称 | Goat Anti-Rabbit IgG H&L antibody |
| 中文名称 | IRDye 800cw羊抗兔IgG (H+L)(进口分装) |
| 别 名 | IRDye? 800CW Goat anti-Rabbit IgG H&L; Immunoglobulin G; 山羊抗兔IgG H&L (IRDye? 800CW)预吸附二抗; ?IRDye 800cw 羊抗兔IgG (H+L); |
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Specific References (9) | bs-40295G-IRDye8 has been referenced in 9 publications.
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| 抗体来源 | Goat |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rabbit |
| 产品应用 | WB=1:10000-30000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 1.0 mg/ml |
| 免 疫 原 | Native Rabbit IgG |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
IRDye800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed Producer: Li-Cor(USA) Product Number: 926-32211 Fluorophore: IRDye 800CW(MW: 1166) Excitation Wavelength: 778nm(in PBS) Emission Wacelegth: 795nm(in PBS) Fluorophore/Protein Ratio: 1.7 moles IRDye 800CW: 1mole IgG IRDye 800CW antibodies can be used for a variety of applications, including Western blotting, In-Cell Western assays, In-Gel Westerns, and many others. Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western applications. |
| 产品图片 |
Sample:
Lane 1,4,7: Mouse Testis tissue lysates
Lane 2,5,8: Rat Testis tissue lysates
Lane 3,6,9: Human MCF-7 cell lysates
Primary: Anti-beta-Actin (bs-0061R) at 1/1:2000 dilution
Secondary: IRDye800CW Goat anti-Rabbit IgG (bs-40295G-IRDye8) at 1:50000~200000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
