| 产品编号 | bs-10711R |
| 英文名称 | Rabbit Anti-DARC antibody |
| 中文名称 | Duffy血型趋化因子受体抗体 |
| 别 名 | CCBP1; CD234; CD234 antigen; DARC; Dfy; Duffy antigen/chemokine receptor; Duffy blood group; DUFFY_MOUSE; FY antibody Fy glycoprotein; Glycoprotein D; GPD; GpFy; Human Duffy blood group antigen (Fya-b+) mRNA complete cds; Plasmodium vivax receptor; WBCQ1; DUFFY_MOUSE. |
| 研究领域 | 心血管 细胞生物 免疫学 转录调节因子 趋化因子 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 37kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DARC: 61-150/336 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. Function: Non-specific receptor for many chemokines. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in liver and brain. Similarity: Belongs to the G-protein coupled receptor Duffy family. SWISS: Q16570 Gene ID: 2532 Database links: Entrez Gene: 2532 Human Entrez Gene: 13349 Mouse Omim: 110700 Human SwissProt: Q16570 Human SwissProt: Q9QUI6 Mouse Unigene: 153381 Human Unigene: 6393 Mouse |
| 产品图片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DARC Polyclonal Antibody, Unconjugated(bs-10711R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DARC Polyclonal Antibody, Unconjugated(bs-10711R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
