| 产品编号 | bs-18539R |
| 英文名称 | Rabbit Anti-CLEC16A antibody |
| 中文名称 | C型凝集素结构域家族16成员A抗体 |
| 别 名 | Protein CLEC16A; C type lectin domain family 16 member A; CL16A; Gop 1; Gop1; CL16A_HUMAN； KIAA0350; MGC111457. |
| 研究领域 | 细胞生物 免疫学 糖尿病 自然杀伤细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Cow) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 118kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CLEC16A |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the C-type lectin domain containing family. Single nucleotide polymorphisms in introns of this gene have been associated with diabetes mellitus, multiple sclerosis and rheumatoid arthritis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] Subcellular Location: Cell membrane, Cytoplasmic. Tissue Specificity: Almost exclusively expressed in immune cells, including dendritic cells, B-lymphocytes and natural killer cells. DISEASE: Insulin-dependent diabetes mellitus is significantly associated with variation within a 233-kb linkage disequilibrium block on chromosome 16p13 that includes KIAA0350. Three common non-coding variants of KIAA0350 in strong linkage disequilibrium reach genome-wide significance for association with the disease. Similarity: Belongs to the CLEC16A/gop-1 family. SWISS: Q2KHT3 Gene ID: 23274 Database links: Entrez Gene: 23274 Human Entrez Gene: 74374 Mouse Omim: 611303 Human SwissProt: Q2KHT3 Human SwissProt: Q80U30 Mouse |
| 产品图片 |
Sample: k562 (human)Cell Lysate at 40 ug
Primary: Anti-CLEC16A(bs-18539R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD
Sample:
K562(Human) Cell Lysate at 30 ug
Siha(Human) Cell Lysate at 30 ug
U-2OS(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- CLEC16A (bs-18539R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD
Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (CLEC16A) Polyclonal Antibody, Unconjugated (bs-18539R) at 1:500 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(bs-18539R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(bs-18539R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
