| 产品编号 | bs-10409R |
| 英文名称 | Rabbit Anti-CCL26 Signal peptide antibody |
| 中文名称 | 嗜酸粒细胞趋化蛋白3抗体 |
| 别 名 | C-C motif chemokine 26; CC chemokine IMAC; CCL 26; CCL26; Chemokine (C C motif) ligand 26; Eotaxin-3; IMAC; Macrophage inflammatory protein 4-alpha; MIP 4a; MIP 4alpha; MIP-4-alpha; MIP4a; MIP4alpha; SCYA 26; SCYA26; Small inducible cytokine A26; Small inducible cytokine subfamily A (Cys Cys) member 26; Small inducible cytokine subfamily A member 26; Small-inducible cytokine A26; Thymic stroma chemokine 1; Thymic stroma chemokine-1; TSC 1; TSC-1; TSC1; CCL26 Signal peptide; . |
| 研究领域 | 细胞生物 免疫学 细胞凋亡 趋化因子 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 11kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat CCL26 Signal peptide: 1-70/94 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009] Function: Chemotactic for eosinophils and basophils. Binds to CCR3. Subunit: Monomer. Subcellular Location: Secreted. Tissue Specificity: Ubiquitously expressed at low levels in various tissues including heart and ovary. Similarity: Belongs to the intercrine beta (chemokine CC) family. SWISS: N/A Gene ID: 292673 Database links: Entrez Gene: 10344 Human Entrez Gene: 541307 Mouse Omim: 604697 Human SwissProt: Q9Y258 Human Unigene: 131342 Human Unigene: 376459 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat urinary bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCL26 Signal peptide) Polyclonal Antibody, Unconjugated (bs-10409R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCL26 Signal peptide) Polyclonal Antibody, Unconjugated (bs-10409R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:U-2OS.
Primary Antibody (green line): Rabbit Anti-CCL26 antibody (bs-10409R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
