| 产品编号 | bs-15392R |
| 英文名称 | Rabbit Anti-GPR91 antibody |
| 中文名称 | G蛋白偶联受体GPR91蛋白抗体 |
| 别 名 | G protein coupled receptor 91; G-protein coupled receptor 91; GPR 91; P2Y purinoceptor 1;P2Y purinoceptor 1-like; Purinergic Receptor P2U2; Succinate receptor; Succinate receptor 1; SUCN R1; SUCNR 1; SUCNR1; SUCR1_HUMAN. |
| 研究领域 | 细胞生物 免疫学 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR91: 131-334/334 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a G-protein-coupled receptor for succinate, an intermediate molecule of the citric acid cycle. It is involved in the promotion of hematopoietic progenitor cell development, and it has a potential role in renovascular hypertension which has known correlations to renal failure, diabetes and atherosclerosis. [provided by RefSeq, Oct 2009] Function: Receptor for succinate. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed specifically in kidney. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q9BXA5 Gene ID: 56670 Database links: Entrez Gene: 56670 Human Entrez Gene: 84112 Mouse Omim: 606381 Human SwissProt: Q9BXA5 Human SwissProt: Q99MT6 Mouse Unigene: 279575 Human Unigene: 125110 Mouse Unigene: 124512 Rat |
| 产品图片 |
Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-GPR91(bs-15392R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kD
Observed band size: 39kD
Protein: 1.kidney lyates(mo);2.liver lyates(mo);
Primary: Rabbit Anti-GPR91 (bs-15392R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:39 kD
Observed band size:49 kD
Western blot analysis of extracts from Liver tissue(mo) using GPR91 Antibody.
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(bs-15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated(bs-15392R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
