| 產品編號 | bs-8615R |
| 英文名稱 | Rabbit Anti-Ribonuclease 3/ECP antibody |
| 中文名稱 | 嗜酸性粒細胞陽離子蛋白抗體 |
| 別 名 | eosinophil cationic protein; EAR; Ear11; Ear4; EAR5; ECP; LOC192264; MGC143624; mR3; mR5; R1; R14; R15; R17; R5; R7; Ribonuclease 3; Rnase17; Rnase2; RNASE3; RNS3; ECP_HUMAN. |
 |
Specific References (4) | bs-8615R has been referenced in 4 publications.
[IF=12.05] Fujisawa, Daisuke, et al. "Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria." Journal of Allergy and Clinical Immunology (2014). IHC-F ; Human.
[IF=4.082] Yoshimasa Imoto. et al. Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis. Biomolecules. 2020 Nov;10(11):1568 IF ; Human.
[IF=2.36] Inada, Noriko, et al. "Histamine H 1 and H 4 receptor expression on the ocular surface of patients with chronic allergic conjunctival diseases." Allergology International (2017). IF(ICC) ; Human.
[IF=2.36] Tokunaga, Takahiro, et al. "The significant expression of TRPV3 in nasal polyps of eosinophilic chronic rhinosinusitis." Allergology International (2017). IHC-P ; Human.
|
| 研究領域 | 細胞生物 免疫學 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,ICC/IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 16kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ribonuclease 3: 45-160/160 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
ECP is a 160 amino acid potent cytotoxic secretory protein that belongs to the pancreatic ribonuclease family. ECP localizes to the granule matrix of the eosinophil and is involved in the immune response system. ECP is a cytotoxin, neurotoxin and helminthotoxin that is secreted by activated human eosinophils. ECP exhibits anti-parasitic, antibacterial and ribonucleolytic activities. It has been suggested that ECP induces the neurotoxic effect known as the Gordon phenomenon, a syndrome manifested by ataxia, muscular rigidity, paralysis, and tremor that may lead to death. ECP is considered a marker of eosinophilic inflammation and high levels have been found in cases of active asthma and other allergic diseases Function: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity. Subcellular Location: Cytoplasmic granule. Matrix of eosinophil's large specific granule. Similarity: Belongs to the pancreatic ribonuclease family. SWISS: P12724 Gene ID: 6037 Database links: Entrez Gene: 6037 Human Entrez Gene: 53876 Mouse Omim: 131398 Human SwissProt: P12724 Human SwissProt: O35290 Mouse Unigene: 73839 Human Unigene: 327386 Mouse Unigene: 10626 Rat |
| 產品圖片 |
Western blot analysis of lysate from human spleen tissue lysate, using RNASE3 Antibody (C-term)(Cat. #bs-8615R). AP13557b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
RNASE3 Antibody (C-term) (Cat. #bs-8615R) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNASE3 antibody detected the RNASE3 protein (arrow).
Confocal immunofluorescent analysis of RNASE3 Antibody (C-term) (Cat#bs-8615R) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
