| 產品編號 | bs-12559R |
| 英文名稱 | Rabbit Anti-B7-H6 antibody |
| 中文名稱 | B7H6蛋白抗體 |
| 別 名 | B7 homolog 6; B7-H6; B7H6; B7H6_HUMAN; DKFZp686O24166; Natural cytotoxicity triggering receptor 3 ligand 1; NCR3LG1; Putative Ig like domain containing protein DKFZp686O24166/DKFZp686I21167. |
| 研究領域 | 細胞生物 免疫學 染色質和核信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human B7-H6: 51-150/454 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3; MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011]. Function: Triggers NCR3-dependent natural killer cell activation. Subunit: Monomer. Interacts specifically with NCR3, but not with other natural killer cell-activating receptors, including NCR1, NCR2 and KLRK1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level). Similarity: Contains 1 Ig-like C1-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q68D85 Gene ID: 374383 Database links: Entrez Gene: 374383 Human Omim: 613714 Human SwissProt: Q68D85 Human Unigene: 146274 Human |
| 產品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- B7-H6 (bs-12559R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Sample:
Huh7 Cell (Human) Lysate at 40 ug
A549 Cell (Human) Lysate at 40 ug
Primary: Anti-B7-H6 (bs-12559R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- B7-H6 (bs-12559R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B7-H6) Polyclonal Antibody, Unconjugated (bs-12559R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
