| 产品编号 | bs-2967R |
| 英文名称 | Rabbit Anti-MBP tag antibody |
| 中文名称 | 麦芽糖结合蛋白抗体 |
| 别 名 | MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein; MALE_ECOLI. |
| 产品类型 | 标签抗体 |
| 研究领域 | 细胞生物 免疫学 糖蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Recombinant protein |
| 产品应用 | WB=1:5000-20000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein: 1-100/396 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein. Function: Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody. Subcellular Location: Periplasm. Similarity: Belongs to the bacterial solute-binding protein 1 family. SWISS: P0AEX9 Gene ID: 1039564 Database links: Entrez Gene: 1039564 Escherichia coli Entrez Gene: 4494002 Escherichia coli Entrez Gene: 948538 Escherichia coli Entrez Gene: 960089 Escherichia coli SwissProt: P0AEX9 Escherichia coli SwissProt: P0AEY0 Escherichia coli |
| 产品图片 |
Sample:
Lane 1: MBP-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33131P) at 4 ug
Primary:
Anti-MBP tag (bs-2967R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
Sample: Lane 1: MBP tagged cytochrome C (Full Length) Protein (bs-41232P) at 4 ug Lane 2: MBP tagged caspase 3 p17 (143-213) Protein (bs-41233P) at 4 ug Primary: Anti-MBP tag (bs-2967R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60-70 kD Observed band size: 62 kD
Sample:
Lane 1: MBP-Tagged Fusion Protein (bs- 41233P) at 300ng
Primary: Anti-MBP tag (bs-2967R) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 60 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
