| 产品编号 | bs-6030R |
| 英文名称 | Rabbit Anti-CD200 antibody |
| 中文名称 | 膜糖蛋白CD200抗体 |
| 别 名 | CD200 antigen; CD200 molecule; MOX1; MOX2; MRC; MRC OX 2 antigen; My033; OX 2; OX 2 membrane glycoprotein precursor; OX-2 membrane glycoprotein; OX2G; OX2G_HUMAN. |
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Specific References (2) | bs-6030R has been referenced in 2 publications.
[IF=2.8] Clark, David A., et al. "The Receptor for the CD200 Tolerance‐Signaling Molecule Associated with Successful Pregnancy is Expressed by Early‐Stage Breast Cancer Cells in 80% of Patients and by Term Placental Trophoblasts." American Journal of Reproductive Immunology 74.5 (2015): 387-391. IHC-P ; Human.
[IF=2.8] Clark, David A., Jorge L. Arredondo, and Sukhbinder Dhesy-Thind. "The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks’ gestation." Journal of Reproductive Immunology 112 (2015): 20-23. IHC-P ; Human.
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| 研究领域 | 细胞生物 免疫学 细胞表面分子 糖蛋白 淋巴细胞 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28kDa |
| 检测分子量 | 40-50 |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD200: 41-140/278 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] Function: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. Subunit: Interacts with CD200R1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P41217 Gene ID: 4345 Database links: Entrez Gene: 4345 Human Omim: 155970 Human SwissProt: P41217 Human Unigene: 79015 Human CD200是Ⅰ型膜糖蛋白，为免疫球蛋白超家族的新成员，广泛表达在人类胸腺细胞、B细胞、激活的T细胞、滤泡状树突细胞、神经元及血管内皮上。可表达于B细胞及其增殖性疾病和一部分T细胞。近年来研究发现，CD200与CD200R间的相互作用可以传递抑制性信号减低髓样细胞的活性，改变其迁移。CD200在免疫疾病和抑制排斥反应中作为一种免疫耐受信号，它的一些相关分子在免疫调节中也具有一定的作用。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (bs-6030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (bs-6030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD200) Polyclonal Antibody, Unconjugated (bs-6030R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD200 Polyclonal Antibody, Unconjugated(bs-6030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD200 Polyclonal Antibody, Unconjugated(bs-6030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
