| 产品编号 | bs-3831R |
| 英文名称 | Rabbit Anti-Adipose Triglyceride Lipase antibody |
| 中文名称 | 脂肪甘油三酯脂酶抗体 |
| 别 名 | PEDF R; Adipose Triglyceride Lipase; TTS 2.2; PEDFR; PEDF-R; ATGL; ATGL DESNUTRIN; Calcium independent phospholipase A2; Desnutrin; EC 3.1.1.3; IPLA2 zeta; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA; Pigment epithelium derived factor; Pnpla2; Transport secretion protein 2; Transport secretion protein 2.2; Triglyceride hydrolase; TTS2; TTS2.2; ZETA. |
 |
Specific References (2) | bs-3831R has been referenced in 2 publications.
[IF=2.742] Liu, Yanrong. et al. Cinnamaldehyde affects lipid droplets metabolism after adipogenic differentiation of C2C12 cells. MOL BIOL REP. 2022 Dec;:1-7 WB ; Mouse.
[IF=2.532] Chenming Zhang. et al. Transcriptomics and proteomics analysis to explore the mechanism of Yishen Tongluo formula repairing sperm DNA damage in rats. ANDROLOGIA. 2022 Sep;:e14582 WB ; Rat.
|
| 研究领域 | 免疫学 生长因子和激素 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=3ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 55kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ATGL: 401-504/504 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Adipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals. Subunit: Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity. Subcellular Location: Lipid droplet. Cell membrane. Tissue Specificity: Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level). Similarity: Contains 1 patatin domain. SWISS: Q96AD5 Gene ID: 57104 Database links: Entrez Gene: 57104 Human Entrez Gene: 66853 Mouse Omim: 609059 Human SwissProt: Q96AD5 Human SwissProt: Q8BJ56 Mouse Unigene: 654697 Human Unigene: 29998 Mouse Unigene: 19196 Rat Pathway: Glycerolipid metabolism; triacylglycerol degradation. |
| 产品图片 |
Sample:
Lane 1: Mouse Brown fat tissue lysates
Lane 2: Mouse Heart tissue lysates
Primary: Anti-Adipose Triglyceride Lipase (bs-3831R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 52 kDa
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (bs-3831R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
