| 產(chǎn)品編號 | bs-2651R |
| 英文名稱 | ELAVL1 Rabbit pAb |
| 中文名稱 | ELAVL1抗體 |
| 別 名 | HuR/ELAVL1; ELAV(embryonic lethal abnormal vision Drosophila) like 1; ELAV like 1; ELAV-like protein 1; ELAV1; ELAV1_HUMAN; Embryonic lethal abnormal vision drosophila homolog like 1; Hu Antigen R; Hu-antigen R; Hua; HuR; MelG. |
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Specific References (2) | bs-2651R has been referenced in 2 publications.
[IF=11.161] Du, Yanhua. et al. SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression. J EXP CLIN CANC RES. 2022 Dec;41(1):1-16 IHC ; Human.
[IF=2.795] Akira Wagatsuma et al. Cobalt chloride, a chemical hypoxia-mimicking agent, suppresses myoblast differentiation by downregulating myogenin expression. Mol Cell Biochem. 2020 Jul;470(1-2):199-214. WB ; Human.
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| 研究領(lǐng)域 | 染色質(zhì)和核信號 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Sheep,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ELAVL1: 251-326/326 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression. [provided by RefSeq]. Function: Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Subunit: Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Subcellular Location: Cytoplasm. Nucleus. Note=Translocates into the cytoplasm following phosphorylation by MAPKAPK2. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated by MAPKAPK2. Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. Similarity: Belongs to the RRM elav family. Contains 3 RRM (RNA recognition motif) domains. SWISS: Q15717 Gene ID: 1994 Database links: Entrez Gene: 1994 Human Entrez Gene: 15568 Mouse Omim: 603466 Human SwissProt: Q15717 Human SwissProt: P70372 Mouse Unigene: 184492 Human Unigene: 713744 Human Unigene: 119162 Mouse |
| 產(chǎn)品圖片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-ELAVL1 (bs-2651R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Ob
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat s
Tissue/cell: human
carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat se
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-ELAVL1 antibody (bs-2651R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
