| 产品编号 | bs-2646R |
| 英文名称 | Rabbit Anti-LAG3 antibody |
| 中文名称 | 淋巴细胞活化基因-3（CD223）抗体 |
| 别 名 | Lymphocyte activation gene 3 protein; Lymphocyte activation gene 3; CD223; FDC protein; LAG 3; LAG-3; LAG3_HUMAN. |
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Specific References (1) | bs-2646R has been referenced in 1 publications.
[IF=2.976] Chen Y et al. Antitumor effects of the silencing of programmed cell death ligand?1 in colorectal cancer via immunoregulation. (2018) Oncol.Rep. IHC ; Mouse.
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| 研究领域 | 细胞生物 免疫学 自然杀伤细胞 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Horse) |
| 产品应用 | Flow-Cyt=1μg /test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58kDa |
| 检测分子量 | 70 |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LAG-3: 201-300/525 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008] Function: Involved in lymphocyte activation. Binds to HLA class-II antigens. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: On cell surface of activated NK and T-lymphocytes. Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q5BK54 Gene ID: 3902 Database links: UniProtKB/Swiss-Prot: P18627.5 Entrez Gene: 3902 Human Omim: 153337 Human SwissProt: P18627 Human Unigene: 409523 Human |
| 产品图片 |
Blank control:Ctll-2.
Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (bs-2646R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Ctll-2.
Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (bs-2646R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
