| 产品编号 | bs-2484R |
| 英文名称 | Rabbit Anti-CCL27 antibody |
| 中文名称 | 皮肤T细胞虏获趋化因子抗体 |
| 别 名 | ALP; CTACK; C-C motif chemokine 27; CC chemokine ILC; Ccl27; CTAK; Cutaneous T cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESkine; IL 11 R alpha locus chemokine; IL-11 R-alpha-locus chemokine; PESKY; SCYA27; Skinkine; Small inducible cytokine A27; Small-inducible cytokine A27. |
| 研究领域 | 免疫学 趋化因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Cow) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 19kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CCL27: 25-112/164 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation. [provided by RefSeq]. Function: Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10. Subunit: Monomer, dimer, and tetramer. Heparin avidly promotes oligomerization. Subcellular Location: Secreted. Tissue Specificity: Testis, thymus, placenta, ovary and skin. Similarity: Belongs to the intercrine beta (chemokine CC) family. SWISS: Q9Z1X0 Gene ID: 10850 Database links: Entrez Gene: 10850 Human Omim: 604833 Human SwissProt: Q5VZ77 Human SwissProt: Q9Y4X3 Human Unigene: 459590 Human Unigene: 648124 Human CCL27是一种CC型趋化因子，又被称为Skinkine，ESkine和CTACK。CCL27基因位于人类染色体9，它与皮肤上归巢的记忆T淋巴细胞有关，并在T细胞介导的皮肤炎症中起着重要的作用。CCL27表达在许多组织，包括性腺，胸腺，胎盘和皮肤。其配体为CCR10,CCL27与接触性软疣病毒编码产生的蛋白质MC148有较高的同源性，但却与人疱疹病毒8型编码产生病毒巨噬细胞炎症蛋白-Ⅱ竞争结合受体CCR10。 |
| 产品图片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCL27 Polyclonal Antibody, Unconjugated(bs-2484R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
