| 产品编号 | bs-2430R |
| 英文名称 | Rabbit Anti-Apelin Receptor antibody |
| 中文名称 | 血管紧张素II受体样蛋白1抗体 |
| 别 名 | APJ receptor; AGTR L1; AGTRL 1; AGTRL1; Angiotensin II receptor like 1; Angiotensin receptor like 1; Apelin receptor; APJ; B78; FLJ90771; G protein coupled receptor APJ; GPCR34; HG 11; HG11; MGC45246; MSR; msr/apj; Aplnr; Agtrl1; APLNR; FLJ96609; APJ_HUMAN. |
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Specific References (4) | bs-2430R has been referenced in 4 publications.
[IF=3.968] Bingyuan Ji et al. Roles for heterodimerization of APJ and B2R in promoting cell proliferation via ERK1/2-eNOS signaling pathway. Cell Signal. 2020 Sep;73:109671. co-IP ; Human.
[IF=2.976] Cemre Nur Balci. et al. The investigation of apelin and apelin receptor expressions in mouse endometrium during peri-implantation period. ANN ANAT. 2023 Feb;246:152027 WB, IHC ; Mouse.
[IF=1.64] Izgüt-Uysal, V. N., et al. "The effect of apelin on the functions of peritoneal macrophages." Physiological research (2017). IHC ; Rat.
[IF=1.61] Ustunel, Ismail, et al. "The effects of water immersion and restraint stress on the expressions of apelin, apelin receptor (APJR) and apoptosis rate in the rat heart." Acta Histochemica (2014) IHC-P ; Rat.
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| 研究领域 | 心血管 细胞生物 免疫学 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Dog) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1μg/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Apelin receptor: 41-140/380 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq Function: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. Subcellular Location: Cell membrane. Tissue Specificity: Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P35414 Gene ID: 187 Database links: Entrez Gene: 429337 Chicken Entrez Gene: 187 Human Entrez Gene: 23796 Mouse Omim: 600052 Human SwissProt: P35414 Human SwissProt: Q9WV08 Mouse Unigene: 438311 Human Unigene: 29368 Mouse Unigene: 7965 Rat |
| 产品图片 |
Sample:
Lane 1: Heart (Mouse) Lysate at 40 ug
Lane 2: Heart (Rat) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Spinal cord (Mouse) Lysate at 40 ug
Lane 6: Spinal cord (Rat) Lysate at 40 ug
Primary: Anti-Apelin Receptor (bs-2430R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 50 kD
Sample:
Cerebrum(mouse) Lysate at 40 ug
heart(mouse) Lysate at 40 ug
Primary: Anti-Apelin receptor (bs-2430R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 52 kD
Blank control（black line）:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Apelin Receptor antibody (bs-2430R)
Dilution:1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Negative control（white blue line）: PBS
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Positive control(blue): mouse Splenocytes
Isotype Antibody (orenge): Rabbit IgG, Dilution: 1ug in 100 u1 X PBS containing 0.5% BSA
Secondary Antibody(light blue): Goat anti-rabbit IgG-FITC,Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody catalog number (green): bs-2430R, Dilution: 1ug in 100ul 1X PBS containing 0.5% BSA
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
