| 产品编号 | bs-1997R |
| 英文名称 | Rabbit Anti-NCX2 antibody |
| 中文名称 | 钠钙交换蛋白2抗体 |
| 别 名 | SLC8A2; Sodium/calcium exchanger 2; solute carrier family 8 (sodium/calcium exchanger), member 2; Na(+)/Ca(2+)-exchange protein 2; Na+/Ca2+ exchanger 2; sodium/calcium exchanger 2 precursor; Slc8a2; NAC2_HUMAN; SLC8A2. |
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Specific References (4) | bs-1997R has been referenced in 4 publications.
[IF=3.457] Chen M et al.
Protective effects of echinacoside against anoxia/reperfusion injury in H9c2 cells via up-regulating p-AKT and SLC8A3.Biomed Pharmacother. 2018 Aug;104:52-59. WB ; Rat.
[IF=3.201] Zhang J et al. TRPV4 Complexes With the Na/Ca 2 Exchanger and IP 3 Receptor 1 to Regulate Local Intracellular Calcium and Tracheal Tension in Mice. Front. Physiol., 06 December 2019. Co-IP&WB ; Mouse.
[IF=2.959] Zhixiu Xia. et al. NCX3 alleviates ethanol-induced apoptosis of SK-N-SH cells via the elimination of intracellular calcium ions. Toxicol In Vitro. 2021 Apr;72:105104 WB ; Human.
[IF=1.963] Zhixiu Xia. et al. The downregulation of NCXs is positively correlated with the prognosis of stage II–IV colon cancer. World J Surg Oncol. 2021 Dec;19(1):1-14 WB,IHC ; Human.
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| 研究领域 | 细胞生物 免疫学 神经生物学 信号转导 交换蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Dog,GuineaPig,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 98kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NCX2: 601-700/921 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Sodium/calcium exchanger proteins are integral membrane proteins primarily seen in cardiac cells. In cardiac myocytes, the concentration of Ca2+ alternates between low levels during relaxation and high levels during contraction. The Na+/Ca2+ exchanger 1 (NCX1) protein mediates Ca2+ extrusion from cardiac cells during relaxation. Four NCX1 isoforms (NCX1.1, NCX1.3, NCX1.7, and NCX1.10) result from alternate splicing. NCX1 mRNA is present at high levels in the heart, with lower levels present in the brain. NCX2 is most abundantly expressed in brain, in contrast the the broader distribution of NCX1, which is also expressed in heart, kidney, lung, smooth and skeletal muscle. The difference in expression for the transporter subtypes is believed to reflect differences in their functional roles. Regulation mechanisms for these exchanger proteins have not been fully characterized. Function: Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores (By similarity). Subcellular Location: Membrane; Multi-pass membrane protein. Similarity: Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily. Contains 2 Calx-beta domains. SWISS: Q9UPR5 Gene ID: 6543 Database links: UniProtKB/Swiss-Prot: Q9UPR5.2 钠钙交换蛋白又称钠钙交换体，有三种同分异构体,即NCX1、NCX2和NCX3。2型Na-Ca交换蛋白-NCX2, 很多组织中都有表达，分布与胞浆及胞膜。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
