| 产品编号 | bs-0536R |
| 英文名称 | Rabbit Anti-Interferon alpha 2b antibody |
| 中文名称 | 干扰素α2b抗体 |
| 别 名 | Interferon alpha-2b; Alpha 2a interferon; alpha-2a interferon; IFN alpha 2b; IFNA 2; IFNA; INFA 2; INFA2; Interferon alpha 2; Interferon alpha 2 precursor; Interferon alpha 8 precursor; Interferon alpha A; Interferon alpha B; Interferon alpha B2; Interferon alpha-2 precursor; LeIF A; LeIF B; LeIFA; MGC125764; Interferon alpha B; Interferon alpha B2; interferon, alpha 8; LeIF B; MGC125765; IFN-alpha-2; IFNA2_HUMAN; MGC125765. |
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Specific References (1) | bs-0536R has been referenced in 1 publications.
[IF=1.797] Shaaban R et al. Molecular and Biological Characterization of a Prepared Recombinant Human Interferon Alpha 2b Isoform.(2018) Appl. Biochem. Biotechnol. 2018 Oct 18. WB ; Rat.
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| 研究领域 | 肿瘤 细胞生物 免疫学 糖蛋白 细胞因子 干扰素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 19kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IFN alpha 2b |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Interferon alpha 2b is one of the known 23 different variants of IFN-alpha. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. Interferon alpha 2b is a potent immune modulator. It can either inhibit or enhance immune cell activity within the tightly regulated microenvironment of inflammation, depending upon the concentration of the cytokine and the activation stage of the cell. Interferon alpha 2B is currently used as an anticancer drug due to it's antiproliferative activity. Function: Produced by macrophages, IFN-alpha have antiviral activities. Subunit: Interacts with IFNAR2. Subcellular Location: Secreted. Similarity: Belongs to the alpha/beta interferon family. SWISS: P01563 Gene ID: 3440 Database links: Entrez Gene: 3440 Human Omim: 147562 Human SwissProt: P01563 Human Unigene: 211575 Human |
| 产品图片 |
Sample:
Lane 1: Recombinant human Interferon alpha 2 protein, N-His(bs-42261P)
Primary: Anti-Interferon alpha 2b (bs-0536R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 16 kDa
Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Interferon alpha 2b) Polyclonal Antibody, Unconjugated (bs-0536R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IFN-Alpha 2b/Interferon alpha 2/INFA2 Polyclonal Antibody, Unconjugated (bs-0536R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
