| 產品編號 | bs-1728R |
| 英文名稱 | Rabbit Anti-CX3CR1 antibody |
| 中文名稱 | 趨化因子受體1抗體 |
| 別 名 | Beta chemokine receptor-like 1; C X3 C CKR 1; CCRL1; Chemokine C X3 C motif receptor 1; CMK BRL 1; CMKBLR1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3C CKR1; Fractalkine Receptor; GPR13; GPRV28; V28; Chemokine receptor 1(chemokine (C-X3-C motif) receptor 1; C-X3-C CKR-1; CX3C1_HUMAN; CX3CR1; G-protein coupled receptor 13. |
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Specific References (5) | bs-1728R has been referenced in 5 publications.
[IF=11.99] Sun et al. Inflammatory signals from photoreceptor modulate pathological retinal angiogenesis via c-Fos. (2017) J.Exp.Med. 214:1753-1767 IHC ; Mouse.
[IF=5.215] Tianrui Zhang. et al. Daphnetin Improves Neuropathic Pain by Inhibiting the Expression of Chemokines and Inflammatory Factors in the Spinal Cord and Interfering with Glial Cell Polarization. PHARMACEUTICALS-BASE. 2023 Feb;16(2):243 WB ; Rat.
[IF=5.085] Venosa Alessandro. et al. Role of CCR2+ Myeloid Cells in Inflammation Responses Driven by Expression of a Surfactant Protein-C Mutant in the Alveolar Epithelium. Front Immunol. 2021 Apr;12:1348 IHC ; Mouse.
[IF=4.12] Nakai et al. IL-17A induces heterogeneous macrophages, and it does not alter the effects of lipopolysaccharides on macrophage activation in the skin of mice. (2017) Sci.Rep. 7:12473 IHC,WB ; Mouse.
[IF=3.457] Hu A et al. Involvement of stromal cell-derived factor-1α (SDF-1α), stem cell factor (SCF), fractalkine (FKN) and VEGF in TSG protection against intimal hyperplasia in rat balloon injury. Biomed Pharmacother. 2019 Feb;110:887-894. IHC-P ; Rat.
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| 研究領域 | 腫瘤 免疫學 神經生物學 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Cow,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CX3CR1: 151-250/355 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010] Function: Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. Subunit: Interacts with human respiratory syncytial virus (HRSV) protein G; this interaction modulates host immune response. Interacts with HIV-1 envelope polyprotein gp160. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in lymphoid and neural tissues. DISEASE: Age-related macular degeneration 12 (ARMD12) [MIM:613784]: A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P49238 Gene ID: 1524 Database links: Entrez Gene: 1524 Human Entrez Gene: 13051 Mouse SwissProt: P49238 Human SwissProt: Q9Z0D9 Mouse |
| 產品圖片 |
Sample:
Heart(Mouse) Lysate at 40 ug
Heart(Rat) Lysate at 40 ug
Primary: Anti-CX3CR1 (bs-1728R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50'55 kD
Observed band size: 53 kD
Sample:
Lane 1: Mouse Spinal cord tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Spinal cord tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-CX3CR1 (bs-1728R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 47 kDa
Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CX3CR1) Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:Raw264.7.
Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody (bs-1728R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
