| 产品编号 | bs-2410R |
| 英文名称 | Rabbit Anti-SHBG antibody |
| 中文名称 | 雄激素结合蛋白抗体 |
| 别 名 | Androgen binding protein; ABP; MGC126834; MGC138391; SBP; Sex steroid binding protein; SHBG; TEBG; Testis specific androgen binding protein; Testosterone binding beta globulin; Testosterone Estradiol Binding Globulin; Testosterone estrogen binding globulin; UniProtKB/Swiss-Prot: P04278.2; SHBG_HUMAN. |
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Specific References (2) | bs-2410R has been referenced in 2 publications.
[IF=2.86] Liu, Yaping, et al. "Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice." International Journal of Molecular Sciences 17.1 (2016): 96. WB ; Mouse.
[IF=2.299] Jiang Y et al. Transcriptome profile of bovine iPSCs derived from Sertoli Cells. Theriogenology. 2019 Nov 19. pii: S0093-691X(19)30522-9. FCM ; Holstein bull.
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| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 生长因子和激素 内分泌病 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SHBG: 51-150/402 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Sex hormone binding globulin (SHBG) is a glycoprotein synthesized by the liver. Circulating androgen and estrogen concentrations influence SHBG synthesis. Elevated testosterone, for example, causes SHBG synthesis to decrease, whereas high estrogen stimulates SHBG production. The regulation of SHBG synthesis, combined with SHBG's higher affinity for testosterone, impacts bioavailable testosterone levels. SHBG binds up to 98 percent of the steroid hormones in the blood including 5a-dihydrotestosterone (DHT), testosterone and androstenediol with particularly high affinity, and estradiol and estrone with slightly lower affinity. Function: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. Subunit: Homodimer. Subcellular Location: Secreted (By similarity). Note=In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis (By similarity). Tissue Specificity: Isoform 1 and isoform 2 are present in liver and testis. Similarity: Contains 2 laminin G-like domains. SWISS: P04278 Gene ID: 6462 Database links: Entrez Gene: 6462 Human Omim: 182205 Human SwissProt: P04278 Human Unigene: 632235 Human 雄激素结合蛋白ABP是一种由睾丸支持细胞合成和分泌的与雄激素有高度亲和力的蛋白,雄激素是依赖于雄激素结合蛋白起作用的,ABP与睾酮或双氢睾酮结合后，转运至曲细精管，提高雄激素在曲细精管的局部浓度，有利于生精过程。 |
| 产品图片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human U87MG cell lysates
Primary: Anti-SHBG (bs-2410R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 50 kDa
Sample:
Lane1: Testis (Mouse) Lysate at 30 ug
Lane2: Liver (Mouse) Lysate at 30 ug
Primary: Anti-ABP/SHBG(bs-2410R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 40kD
Observed band size : 44kD
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (bs-2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (bs-2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (bs-2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
