| 產(chǎn)品編號(hào) | bs-1475R |
| 英文名稱 | Rabbit Anti-Gremlin antibody |
| 中文名稱 | 骨形態(tài)形成蛋白拮抗蛋白抗體 |
| 別 名 | CKTSF1B1; GREMLIN-1; GREMLIN 1; GREMLIN1; Cysteine knot superfamily BMP antagonist 1; DAND2; DRM; GREMLIN; Gremlin1; Gremlin 1; Gremlin-1; IHG2; PIG2; proliferation inducing gene 2 protein. |
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Specific References (4) | bs-1475R has been referenced in 4 publications.
[IF=3.7] Wellbrock, Jasmin, et al. "Overexpression of Gremlin-1 in Patients with Loeys-Dietz Syndrome: Implications on Pathophysiology and Early Disease Detection." PLoS one 9.8 (2014): e104742. IHC-P ; Human.
[IF=2.094] He H et al. Low oxygen concentrations improve yak oocyte maturation and enhance the developmental competence of preimplantation embryos. Theriogenology. 2020 Oct 15;156:46-58. WB ; Yak.
[IF=1.817] Yoichiro Aoshima. et al. Gremlin-1 for the Differential Diagnosis of Idiopathic Pulmonary Fibrosis Versus Other Interstitial Lung Diseases: A Clinical and Pathophysiological Analysis. 2021 Mar 26 IHC ; Human.
[IF=0] KAWAGUCHI, Kenichiro, et al. "Role of Gremlins in the Aortic Arch of Spontaneously Hypertensive and Hyperlipidemic Rats." The Showa University Journal of Medical Sciences 28.2 (2016): 89-99. Rat.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 發(fā)育生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞 細(xì)胞骨架 細(xì)胞外基質(zhì) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human gremlin: 101-184/184 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
In Xenopus, Gremlin 1 belongs to a novel gene family that act as BMP antagonists in embryonic explants. The human homolog of Gremlin 1(CKTFS1B1) is localized on human chromosome 15q13--> q15. Gremlin 1-specific mRNA is expressed in different human tissues, including brain, ovary, intestine and colon. It has been suggested that down-regulation of Gremlin 1 is associated with tumor progression, and support the hypothesis that human Gremlin 1 may play an important role during both neuroembryological development and carcinogenesis. Function: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. Subunit: Interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. Subcellular Location: Secreted. Tissue Specificity: Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Similarity: Belongs to the DAN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. SWISS: O60565 Gene ID: 26585 Database links: Entrez Gene: 26585 Human Entrez Gene: 23892 Mouse Omim: 603054 Human SwissProt: O60565 Human SwissProt: O70326 Mouse Unigene: 40098 Human Unigene: 166318 Mouse Unigene: 42929 Rat gremlin屬于骨形態(tài)形成蛋白BMP拮抗劑家族成員,在胚胎發(fā)育過程中可通過調(diào)節(jié)BMP活性，影響器官、體征的形成和組織分化。 |
| 產(chǎn)品圖片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Gremlin (bs-1475R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
Sample:
intestine (Mouse) Lysate at 40 ug
skin (Mouse) Lysate at 40 ug
Primary: Anti-Gremlin (Bs-1475R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 24 kD
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Gremlin) Polyclonal Antibody, Unconjugated (bs-1475R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Gremlin) Polyclonal Antibody, Unconjugated (bs-1475R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat rectum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gremlin) Polyclonal Antibody, Unconjugated (bs-1475R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Gremlin Polyclonal Antibody, Unconjugated(bs-1475R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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