| 产品编号 | bs-0976R |
| 英文名称 | Rabbit Anti-LPLUNC1 antibody |
| 中文名称 | 鼻咽癌癌基因抗体 |
| 别 名 | BPI fold containing family B, member 1; long palate, lung and nasal epithelium carcinoma associated 1 isoform 1; chromosome 20 open reading frame 114; C20orf114; Long palate; BPIB1_HUMAN; Lplunc1; lung and nasal epithelium carcinoma-associated protein 1; VEMSGP; Von Ebner minor salivary gland protein; MGC14597; MGC156708; RGD1563047; RP23-154J12.1; U46068. |
| 研究领域 | 免疫学 微生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LPLUNC1: 21-120/484 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene may be involved in the innate immune response to bacterial exposure in the mouth, nasal cavities, and lungs. The encoded protein is secreted and is a member of the BPI/LBP/PLUNC protein superfamily. This gene is found with other members of the superfamily in a cluster on chromosome 20. [provided by RefSeq] Function: May play a role in innate immunity in mouth, nose and lungs. Subcellular Location: Secreted (By similarity). Tissue Specificity: Detected in trachea, nasal septal epithelium and lung. Similarity: Belongs to the BPI/LBP/Plunc superfamily. Plunc family. SWISS: Q8TDL5 Gene ID: 92747 Database links: Entrez Gene: 92747 Human SwissProt: Q8TDL5 Human Unigene: 65551 Human |
| 产品图片 |
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- LPLUNC1 (bs-0976R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- LPLUNC1 (bs-0976R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
Lane1: Lung(Rat) Lysate at 30 ug
Lane2:Colon carcinoma(Human) Lysate at 30 ug
Primary: Anti-LPLUNC1 (bs-0976R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size: 50kD
Observed band size: 53kD
Tissue/cell: human Nasal polyps tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LPLUNC1 Polyclonal Antibody, Unconjugated(bs-0976R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
