| 产品编号 | bs-1390R |
| 英文名称 | Rabbit Anti-Cdc6 antibody |
| 中文名称 | 细胞分裂周期蛋白6抗体 |
| 别 名 | Cell Division Cycle protein 6; Cdc 18L; Cdc 6; CDC18 (cell division cycle 18, S.pombe, homolog) like; CDC18 (S.pombe); CDC18 like; CDC18(S.pombe); Cdc18L; CDC6 related protein; Cdc6p; Cell cycle controller; Cell division control protein 6; Cell division control protein 6 homolog; Cell division cycle 18; Cell division cycle 18 homolog; Cell division cycle 6 homolog; Cell division cycle 6 protein; HsCDC 18; HsCDC 6; HsCDC18; HsCDC6; p62; p62(cdc 6); p62(cdc6). |
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Specific References (2) | bs-1390R has been referenced in 2 publications.
[IF=4.522] Elsaeid Elnour I et al. Bta‐miR‐885 promotes proliferation and inhibits differentiation of myoblasts by targeting MyoD1. J Cell Physiol. 2020 Jan 27. WB ; Bovine.
[IF=1.422] Ran M et al. miR-26a suppresses autophagy in swine Sertoli cells by targeting ULK2.Reprod Domest Anim. 2018 Aug;53(4):864-871. WB ; Pig.
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| 研究领域 | 细胞生物 染色质和核信号 细胞周期蛋白 转录调节因子 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Cow) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cdc6: 151-300/560 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cell cycle events are regulated by the sequential activation and deactivation of cyclin depended kinase (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc6 is essential for assembling the pre-replicative complex that forms at origins of DNA replication in the G1 phase of the cell cycle in budding yeast (S. cerevisiae). Cdc6 is also involved in checkpoint controls that ensure that DNA replication is completed before initiation of mitosis. Function: Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. Subunit: Interacts with PCNA, ORC1L, cyclin-CDK and HUWE1. Subcellular Location: Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells. DISEASE: Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5) [MIM:613805]. MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal. Similarity: Sequence similaritiesBelongs to the CDC6/cdc18 family. SWISS: Q99741 Gene ID: 990 Database links: Entrez Gene: 990 Human Entrez Gene: 23834 Mouse Omim: 602627 Human SwissProt: Q99741 Human SwissProt: O89033 Mouse Unigene: 405958 Human Unigene: 20912 Mouse 转录调节因子（Transcriptin Regulators） 细胞分裂周期蛋白6也是细胞周期调节蛋白，是真核生物DNA复制的主要调控因子,参与构成DNA前复制复合物。 |
| 产品图片 |
Sample: K562 Cell Lysate at 30 ug
Primary: Anti- Cdc6 (bs-1390R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cdc6) Polyclonal Antibody, Unconjugated (bs-1390R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cdc6 Polyclonal Antibody, Unconjugated(bs-1390R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cdc6) polyclonal Antibody, Unconjugated (bs-1390R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Cdc6 antibody (bs-1390R)
Dilution: 1ug/Test;
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
