| 产品编号 | bs-0891R |
| 英文名称 | Rabbit Anti-Pokemon/ZBTB7A antibody |
| 中文名称 | 扑克蒙蛋白抗体 |
| 别 名 | Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI-1; FBI1; HIV-1 1st-binding protein 1; Leukemia/lymphoma related factor; LRF; Pokemon; TIP21; TTF-I interacting peptide 21; ZBTB7; ZBTB7A; Zinc finger and BTB domain-containing protein 7A; ZBT7A_HUMAN. |
 |
Specific References (1) | bs-0891R has been referenced in 1 publications.
[IF=2.06] Zhao, Yi, et al. "Pokemon enhances proliferation, cell cycle progression and anti-apoptosis activity of colorectal cancer independently of p14ARF–MDM2–p53 pathway." Medical Oncology 31.12 (2014): 1-8. IHC-P ; Human.
|
| 研究领域 | 肿瘤 免疫学 微生物学 转录调节因子 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Pokemon: 151-250/569 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy. Function: Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'. Subunit: Interacts with BCL6. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level). Similarity: Contains 1 BTB (POZ) domain. Contains 4 C2H2-type zinc fingers. SWISS: O95365 Gene ID: 51341 Database links: Entrez Gene: 51341 Human Entrez Gene: 16969 Mouse Omim: 605878 Human SwissProt: O95365 Human SwissProt: O88939 Mouse Unigene: 591384 Human Unigene: 20920 Mouse Pokemon(7APOK Erythroid Myeloid Ontogenic factor)能够控制将正常细胞变成癌细胞所需的途径,Pokemon蛋白是转录因子蛋白家族的一员，并且在人类癌症中发生了突变。另外Pokemon蛋白作为转录因子参与一些细胞基因转录的调节,并在细胞分化过程中发挥着关键、多效性的功能。近来发现,Poke-mon在致癌转化过程中发挥着至关重要的功能,并与肿瘤的发生密切相关。 这种蛋白很可能在实质肿瘤中处于重要地位,还有学者认为：Pokemon在一定类型的B细胞和T细胞淋巴瘤中水平非常高，并发现具有高水平Pokemon蛋白表达对肿瘤恶性程度改变更大。 主要定位于细胞核。 扑克蒙”蛋白又称“波克曼”蛋白。 |
| 产品图片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-Pokemon/ZBTB7A (bs-0891R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-Pokemon/ZBTB7A (bs-0891R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-Pokemon/ZBTB7A (bs-0891R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Tissue/cell: human gratis carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Pokemon Polyclonal Antibody, Unconjugated(bs-0891R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Pokemon Polyclonal Antibody, Unconjugated(bs-0891R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
