| 产品编号 | bs-1296R |
| 英文名称 | Rabbit Anti-Defensin beta 2 antibody |
| 中文名称 | 防御素β2/Defensin β2抗体 |
| 别 名 | beta-defensin 2 precursor; beta Defensin 2; beta-defensin 2; Defb2; Q32ZI5_RAT; DFB4A_HUMAN. Defensin β2. |
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Specific References (2) | bs-1296R has been referenced in 2 publications.
[IF=3.365] Shalong Wang. et al. Bovine Lactoferrin Protects Dextran Sulfate Sodium Salt Mice Against Inflammation and Impairment of Colonic Epithelial Barrier by Regulating Gut Microbial Structure and Metabolites. Front Nutr. 2021; 8: 660598 WB,IF ; Mouse.
[IF=2.245] Renli Qi. et al. Changes of Gut Microbiota and Its Correlation With Short Chain Fatty Acids and Bioamine in Piglets at the Early Growth Stage. Front Vet Sci. 2020; 7: 617259 WB ; Pig.
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| 研究领域 | 微生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 8kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat beta 2 Defensin: 21-71/71 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Jul 2008] Subcellular Location: Secreted. Tissue Specificity: Expressed in the skin and respiratory tract. Similarity: Belongs to the beta-defensin family. LAP/TAP subfamily. SWISS: Q32ZI5 Gene ID: 641631 Database links:
防御素在人体中广泛分布的，目前已知的α－防御素有6种，研究人员从人体的肾脏、气管、鼻粘膜与唾液中也发现了两种β防御素，在许多器官的上皮细胞与非上皮组织的细胞中发现了β－防御素。 Defensin β2在维持肌体天然免疫功能、介导炎症反应以及调节特异性免疫中的作用引起人们的重视，防御素的表达与病原体的入侵紧密相关。 |
| 产品图片 |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-beta 2 Defensin Polyclonal Antibody, Unconjugated(bs-1296R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
