| 產(chǎn)品編號 | bs-0478R |
| 英文名稱 | Rabbit Anti-SLC6A19 antibody |
| 中文名稱 | 鉀依賴鈉鈣交換蛋白6抗體 |
| 別 名 | B(0)AT1; Sodium dependent neutral amino acid transporter B(0); Solute carrier family 6 member 19; System B(0) neutral amino acid transporter. |
| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 通道蛋白 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLC6A19: 31-100/634 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Transporter that mediates epithelial resorption of neutral amino acids across the apical membrane of epithelial cells in the kidney and intestine. It appears that leucine is the preferred substrate, but all large neutral non-aromatic L-amino acids bind to this transporter. Uptake of leucine is sodium-dependent. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent. Subcellular Location: Membrane; Multi-pass membrane protein (Probable). Tissue Specificity: Abundantly expressed in kidney, but not in intestine. Similarity: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A19 subfamily. SWISS: Q695T7 Gene ID: 340024 Database links: Entrez Gene: 340024 Human SwissProt: Q695T7 Human 主要用于：胰島素瘤細(xì)胞與胰島素分泌關(guān)系方面的研究，主要在人主動脈內(nèi)皮細(xì)胞中表達(dá),并且受到葡萄糖的調(diào)控。 |
| 產(chǎn)品圖片 |
Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SLC6A19 Polyclonal Antibody, Unconjugated(bs-0478R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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