| 產品編號 | bs-0015R |
| 英文名稱 | IGF2 Rabbit pAb |
| 中文名稱 | 胰島素樣生長因子-II抗體 |
| 別 名 | IGF2_HUMAN; Insulin-like growth factor II; IGF-II; IGF II; Somatomedin-A; T3M-11-derived growth factor; Insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; Preptin; PP1446; Insulin like Growth Factor 2; |
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Specific References (4) | bs-0015R has been referenced in 4 publications.
[IF=5.162] Lei Zhao. et al. Proteomic analysis reveals the molecular mechanism of Hippophae rhamnoides polysaccharide intervention in LPS-induced inflammation of IPEC-J2 cells in piglets. Int J Biol Macromol. 2020 Dec;164:3294 WB ; Pig.
[IF=4.932] Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFκB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096 WB ; Human.
[IF=2.426] Yuanyuan Wang. et al. Role of AURKA in the hypothalamus–pituitary–testicular axis in Tibetan sheep from Tianzhu. Gen Comp Endocr. 2021 Jan;300:113617 WB ; Sheep.
[IF=0] Ma Y et al. Promotion of Insulin-like growth factor II in cell proliferation and epithelial–mesenchymal transition in hepatocellular carcinoma.J Cancer Res Ther. 2018;14(4):844-850. WB ; Human.
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| 研究領域 | 免疫學 發育生物學 信號轉導 生長因子和激素 糖尿病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 7.4 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IGF2: 25-51/180 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The IGF2 gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is expressed only from the paternally inherited allele and is an imprinted gene which is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types. Function: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. Subcellular Location: Secreted. Post-translational modifications: O-glycosylated with core 1 or possibly core 8 glycans. Thr-96 is a minor glycosylation site compared to Thr-99. DISEASE: Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS) [MIM:180860]. A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Similarity: Belongs to the insulin family. SWISS: P01344 Gene ID: 3481 Database links: Entrez Gene: 3481 Human Entrez Gene: 16002 Mouse SwissProt: P01344 Human SwissProt: P09535 Mouse 胰島素樣生長因子2(IGF-2又稱Somatomedin A)，是一種生長調節激素，由肝分泌并入血液循環的中性多肽，具有調節生長和代謝、胰島素樣及促細胞分裂的活性，為主要的胚胎生長因子。 |
| 產品圖片 |
Tissue/cell: rat placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGF II Polyclonal Antibody, Unconjugated(bs-0015R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF II) Polyclonal Antibody, Unconjugated (bs-0015R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-IGF II antibody (bs-0015R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
