| 产品编号 | bs-0062R |
| 英文名称 | Rabbit Anti-Calretinin antibody |
| 中文名称 | 钙结合蛋白抗体 |
| 别 名 | 29 kDa calbindin; CAB 29; CAB29; CAL 2; CAL2; CALB 2; CALB2; Calbindin 2 29kDa; Calbindin 2; Calbindin D29K; Calbindin2; CR; CALB2_HUMAN; Calretinin. |
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Specific References (1) | bs-0062R has been referenced in 1 publications.
[IF=3.9] Suzui, Masumi, et al. "Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors." Cancer Science 107.7 (2016): 924-935. IHC-P ; Rat.
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| 研究领域 | 神经生物学 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 29kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calretinin: 211-271/271 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Calretinin is a calcium-binding protein which is abundant in auditory neurons. It belongs to the calbindin family. Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin1. Function: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Tissue Specificity: Brain. Similarity: Belongs to the calbindin family. Contains 6 EF-hand domains. SWISS: P22676 Gene ID: 794 Database links: Entrez Gene: 396255 Chicken Entrez Gene: 794 Human Entrez Gene: 12308 Mouse Entrez Gene: 393684 Zebrafish Omim: 114051 Human SwissProt: P07090 Chicken SwissProt: P22676 Human SwissProt: Q08331 Mouse Unigene: 106857 Human Unigene: 2755 Mouse Unigene: 10321 Rat 钙结合蛋白(Calretinin,CR)主要存在于神经组织中，近10%的腺癌可以表达此蛋白的局部染色阳性，而绝大多数的间皮瘤为阳性表达，因此它是诊断间皮瘤的一个有用的标记物 。 钙结合蛋白是平滑肌和非肌性调控蛋白。它可以与肌动蛋白、肌球蛋白、原肌球蛋白和钙调素相互作用。 |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Rat Cerebrum Lysates
Primary: Anti- Calretinin (bs-0062R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Sample:
U-87MG(Human) Cell Lysate at 30 ug
Primary: Anti-Calretinin (bs-0062R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Calretinin/CA Polyclonal Antibody, Unconjugated(bs-0062R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-S100A13 antibody (bs-2617R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
