| 产品编号 | bs-1035R |
| 英文名称 | Rabbit Anti-CD86 antibody |
| 中文名称 | CD86抗体 |
| 别 名 | CD28LG2; FUN1; LAB72; Activation B7 2 antigen; Activation B7-2 antigen 3; CD86_HUMAN; Activation B7-2 antigen; Activation B72 antigen; B lymphocyte activation antigen B7 2; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2 2; B-lymphocyte activation antigen B7-2; B7 2; B7 2 antigen; B7; B7-2; B7.2; B70; B72 antigen; BU63; CD 86; CD28 antigen ligand 2 2; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2; CD86 antigen; CD86 molecule; CLS1; CTLA 4 counter receptor B7 2; CTLA 4 counter receptor B7.2; CTLA-4 counter-receptor B7.2 2, 3; CTLA4 counter receptor B72; Early T-cell costimulatory molecule 1; ETC-1; FUN 1; LAB72; Ly-58; Ly58; MB7; MB7-2; Membrane glycoprotein; MGC34413; T lymphocyte activation antigen CD86; T-lymphocyte activation antigen CD86; TS/A-2. |
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Specific References (63) | bs-1035R has been referenced in 63 publications.
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| 研究领域 | 免疫学 微生物学 干细胞 细胞表面分子 糖蛋白 淋巴细胞 t-淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31kDa |
| 检测分子量 | 72-74 |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of rat CD86: 140-175/313 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]. Function: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. Subunit: Homodimer. Interacts with MARCH8. Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with adenovirus subgroup B fiber proteins and acts as a receptor for these viruses. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed by activated B-lymphocytes and monocytes. Post-translational modifications: Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: O35531 Gene ID: 56822 Database links: Entrez Gene: 942 Human Omim: 601020 Human SwissProt: P42081 Human Unigene: 171182 Human Unigene: 229570 Rat CD86是一个很重要要的辅助刺激分子，CD86作为CD28的辅助刺激分子，提供 T 细胞活化的辅助信号,它们之间的结合和随后介导的信号转导是 T 细胞和 APC 相互协作的重要分子基础。 |
| 产品图片 |
Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: U937 (Human) Cell Lysate at 30 ug
Lane 3: Spleen (Rat) Lysate at 40 ug
Lane 4: Spleen (Mouse) Lysate at 40 ug
Lane 5: HL-60 (Human) Cell Lysate at 30 ug
Primary: Anti-CD86 (bs-1035R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72-74 kD
Observed band size: 72 kD
Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated(bs-1035R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated(bs-1035R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
