| 产品编号 | bs-1571R |
| 英文名称 | Rabbit Anti-F-Actin antibody |
| 中文名称 | 纤维状肌动蛋白抗体 |
| 别 名 | f actin; Filamentous actin; F-actin capping protein alpha subunit; CapZ alpha-1; CAZA1_HUMAN; CAPZA1. |
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Specific References (31) | bs-1571R has been referenced in 31 publications.
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| 研究领域 | 免疫学 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow) |
| 产品应用 | WB=1:1000-5000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=3μg /test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Synthetic MAP peptide derived from human beta-Actin: 1-50/374 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins, found in muscletissues, are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Individual subunits of microfilaments are known as globular actin (G-actin). G-actin subunits assemble into long filamentous polymers called F-actin. SWISS: P60709 Gene ID: 60 Database links: Entrez Gene: 60 Human Entrez Gene: 11461 Mouse Entrez Gene: 100009272 Rabbit Omim: 102630 Human SwissProt: P60709 Human SwissProt: P60710 Mouse SwissProt: P29751 Rabbit Unigene: 520640 Human Unigene: 708120 Human Unigene: 727576 Human Unigene: 328431 Mouse Unigene: 391967 Mouse Unigene: 94978 Rat 纤维状肌动蛋白F-Actin主要存在于横纹肌原肌纤维细丝及平滑肌中，是肌肉运动和细胞运动的重要蛋白。 |
| 产品图片 |
Sample:
Lane1:Hela (Human) Whole Cell Lysate at 30 ug
Lane2:Jurkat (Human) Whole Cell Lysate at 30 ug
Lane3:293T (Human) Whole Cell Lysate at 30 ug
Lane4:Hepg2 (Human) Whole Cell Lysate at 30 ug
Lane5:A431 (Human) Whole Cell Lysate at 30 ug
Lane6:A549 (Human) Whole Cell Lysate at 30 ug
Lane7:H9C2 (Rat) Whole Cell Lysate at 30 ug
Lane8:Testis (Mouse) Tissue Lysate at 30 ug
Primary: Anti-F-Actin(bs-1571R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti- F-Actin antibody (bs-1571R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
